학술논문
전기방사를 이용한 양막의 제조 및 특성 / Preparation and Characterization of the Reconstructed Amniotic Membrane by Electrospinning
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
English
Abstract
양막 안에는 많은 수의 성장인자 (예를들어: EGF, VEGF, HGF, bFGF)가 포함되어 있기 때문에 상처치료 드레싱을 포함한 다양한 의료용으로써 사용되어 왔다. 하지만 양막은 상업적으로 사용하기 비싸다는 한계가 있어, 본 실험에서는 전기방사 기술을 이용하여 부직포로 만들어 양막을 재구성 했다. 중요한 성장인자(bFGF)를 포함하는 가용화 된 양막을 얻기 위해 최적의 방법으로 활용하였다. 가용화된 양막은 기계적 강도를 높이기 위하여 합성폴리머 Poly-urethane과 같이 공방사 하여, 기계적 강도가 향상되었음을 알 수 있었다. 나노섬유 부직포의 형태는 SEM을 통하여서 검사하였고, 재구성 된 양막 부직포의 세포독성은 MTT assay를 통하여 확인하였다. 양막 함유 드레싱제제의 동물 임상 실험 결과 3개의 다른 농도(2.5%, 3.5%, 4.5%)를 함유한 드레싱제제를 창상 유도 후 0-14 일 까지 평가한 결과 2.5% 와 3.5 % 적용 군에서는 현저히 대조군들에 비해서 재생된 상기저층, 진피층이 정상조직과 유사하게 재생되었다. 한편 4.5% 적용군에서는 표피층의 기저세포의 과다증식과 진피층의 콜라겐 밀도가 정상조직에 비해서 높아진 것이 관찰되었다. 재구성 된 양막 2.5%와 3.5% 드레싱이 상처드레싱으로서 우수함을 확인하였다.
Amniotic membrane (AM) has been used for various medical applications including wound dressing, because of a number of growth factors such as EGF, VEGF, HGF and bFGF incorporated in AM. However, High cost of AM has limited the commercial applications. In this study, AM was reconstructed by preparing non-woven matrix by electrospinning technique. In order to verify the properties of reconstructed AM nanofiber scaffold, we performed bFGF release test, scanning electron microscopy, contact angle, water uptake, water vapor permeability, mechanical property, and cellular test. Solubilized AM was co-electrospun with synthetic polymer like PU. Resulting matrix showed an improved mechanical strength. The nano-fiber matrix was examined by SEM. According to increase of AM amount, water contact angle are increased. In contrast, increase of the AM amount diminished water-uptake ratio and water vapor permeability (WVP). However, the WVP of all the reconstructed AMs is within the ideal range for dressing. Effect of reconstruction AM on cytotoxicity was confirmed by MTT assay. When the amount of AM increased, the cell proliferation increased with the same amount of culture time. Resulting reconstruction AM is expected to be an excellent wound dressing. Results of animal test, PU-coll/AM 2,5% and 3,5% groups underwent nearly total epithelialization, showing rete ridges. PU-coll/AM 4.5% and AmniSite-BA were observed excessive proliferation in stratum basale of epidermis.
Amniotic membrane (AM) has been used for various medical applications including wound dressing, because of a number of growth factors such as EGF, VEGF, HGF and bFGF incorporated in AM. However, High cost of AM has limited the commercial applications. In this study, AM was reconstructed by preparing non-woven matrix by electrospinning technique. In order to verify the properties of reconstructed AM nanofiber scaffold, we performed bFGF release test, scanning electron microscopy, contact angle, water uptake, water vapor permeability, mechanical property, and cellular test. Solubilized AM was co-electrospun with synthetic polymer like PU. Resulting matrix showed an improved mechanical strength. The nano-fiber matrix was examined by SEM. According to increase of AM amount, water contact angle are increased. In contrast, increase of the AM amount diminished water-uptake ratio and water vapor permeability (WVP). However, the WVP of all the reconstructed AMs is within the ideal range for dressing. Effect of reconstruction AM on cytotoxicity was confirmed by MTT assay. When the amount of AM increased, the cell proliferation increased with the same amount of culture time. Resulting reconstruction AM is expected to be an excellent wound dressing. Results of animal test, PU-coll/AM 2,5% and 3,5% groups underwent nearly total epithelialization, showing rete ridges. PU-coll/AM 4.5% and AmniSite-BA were observed excessive proliferation in stratum basale of epidermis.