학술논문
UPLC-PDA 및 LC-QTOF에 의한 식이보충제에서 생리활성화합물들의 동시분석 방법 개발 및 검증 / Development and validation of a simultaneous analytical method for bioactive compounds in dietary supplement by UPLC-PDA and LC-QTOF
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
Korean
Abstract
생리활성물질은 인간에게 다양하고 중요한 생리적 영향을 미치고 식이보충제에 포함될 수 있으며 이는 식품에서는 사용이 금지된 성분이다. 적절한 의학적 처방 없이 식이보충제를 통한 생리활성물질의 남용은 생명을 위협하는 부작용을 일으킬 수 있다. 본 연구는 식이보충제에서 생리활성물질 8종을 동시에 스크리닝하고 정량할 수 있는 분석방법을 개발하고 검증하기 위해 수행되었다. UPLC-PDA를 사용하여 식이보충제에서 8가지 생리활성물질(Salicin, Echinacoside, Huperzine A, Magnoflorine, Hydrastine, Canadine, Berberine, α-Asarone)의 정확하고 간단하며 빠른 동시분석 방법을 개발하고 검증하였다. 개발된 방법은 완전히 검증되었으며 특이성, 직선성(r²>0.99), 검출한계(0.08 μg/mL), 정량한계(0.27 μg/mL), 정밀도(RSD
Bioactive compounds have diverse and important physiological effects on humans and can be included in dietary supplements, which are prohibited for use in food. An abuse of bioactive compounds via dietary supplements without proper medical prescription can cause life-threatening side effects. This study was conducted to develop and validate analytical methods that can simultaneously screen and quantify 8 bioactive compounds in dietary supplements. We developed an accurate, simple, rapid and simultaneous analysis method of eight bioactive compounds(Salicin, Echinacoside, Huperzine A, Canadine, Berberine, α-Asarone) in dietary supplements using Ultra Performance Liquid Chromatography coupled with PhotoDiode Array detection (UPLC/PDA). The developed method was fully validated and showed good results with respect to specificity, linearity(r²>0.99), limit of detection (0.08 μg/mL), limit of quantification(0.27 μg/mL), precision (RSD
Bioactive compounds have diverse and important physiological effects on humans and can be included in dietary supplements, which are prohibited for use in food. An abuse of bioactive compounds via dietary supplements without proper medical prescription can cause life-threatening side effects. This study was conducted to develop and validate analytical methods that can simultaneously screen and quantify 8 bioactive compounds in dietary supplements. We developed an accurate, simple, rapid and simultaneous analysis method of eight bioactive compounds(Salicin, Echinacoside, Huperzine A, Canadine, Berberine, α-Asarone) in dietary supplements using Ultra Performance Liquid Chromatography coupled with PhotoDiode Array detection (UPLC/PDA). The developed method was fully validated and showed good results with respect to specificity, linearity(r²>0.99), limit of detection (0.08 μg/mL), limit of quantification(0.27 μg/mL), precision (RSD