부산대학교 도서관

Guided bone regeneration (GBR) is a common procedure for the treatment of bone defects and bone augmentation. Using barrier membrane, GBR is now extensively studied and good result was reported since it's introduction in the late 1980s. In the respect of GBR, barrier membrane creates isolated space surround bone and induces cell differentiation and migration. Nanofibrous structure of electrospinning technique is a successful key for GBR. Because it is to mimic the natural extracellular matrix and porous structure. and it has good flexibility, adhesion to cell also. Polytetrafluoroethylene (PTFE) is fluorine resin polymer and considered as promising membrane due to chemical inactivity, high inviscosity, heat stability, and low coefficient. Collagen is one of most abundant protein in mammals and its role is essential to our way of life. Above all, collagen-based scaffolds have been demonstrated by tissue engineering applications. Alginate is polymer with excellent properties like cellulose, chitin and has biodegradability, biocompatibility, environment-friendless. In particular, alginate in combination with sodium, has an economical advantage and water soluble properties. for these reasons, Alginate is possible to various type of production, such as Nanofiber, Particle, Membrane and so on. Gelatin also present in the transformed triple helix single-stranded structure, in the form of collagen, a natural polymer, such as alginate. Gelatin is commonly known as polymers, which are widely spread in many biological tissue engineering and biocompatibility due to the ease of processing. In this study, we fabricated a barrier membrane that was PTFE membrane and Alginate/gelatin-Collagen double-layered membrane including nano to micro size scale via electrospinning method. Characterization of these barrier membrane by SEM, ATR-FTIR, Contact angle, XRD, Porosity, XPS, BET theory method. These membrane were examined to cell growth activation from osteoblast and fibroblast cell culture by MTT assay with SEM assessment of cell growth, ALP activity, IL-6 expression, ARS staining.