학술논문
Hot-water Extract of S.crispa Has Anti-adipogenic Effects in 3T3-L1 Cell and Anti-obesity Effects in ob/ob mice
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
English
Abstract
꽃송이버섯 (Sprasis crispa)은 생리활성 물질로 콜레스테롤 저하 효능을 가진 β-1,3-glucan을 풍부하게 함유하고 있다. 하지만 최근까지 알려진 꽃송이버섯 연구에서는 항암 효과와 관련된 연구만이 입증 되었다. 이에 본 연구에서는 β -1,3-glucan의 성분을 월등히 함유하고 있는 꽃송이버섯의 항 비만에 관련된 효능을 관찰하였다. 즉, 꽃송이버섯 열수추출물이 3T3-L1 지방세포에서 지방축적 억제효과와 비만형질 마우스 (ob/ob) 에서 체중 및 에너지 대사에 미치는 영향을 알아보고자 하였다. 꽃송이버섯으로부터 추출한 열수추출물은 지방세포 분화 과정에서 중성지방 축적이 농도별, 시간별 의존적으로 감소하였고 글리세롤 분비량은 농도별, 시간별 의존적으로 증가하는 것을 확인하였다. 지방생성 조절 인자인 C/EBP β와 PPAR γ 의 발현량 또한 감소한 것을 확인 할 수 있었다. 또한, 10주간 고지방 식이를 공급한 ob/ob 마우스 모델에서 꽃송이버섯 추출물을 처리하였을 때, 체중 증가량과 식이효율이 대조군에 비해 현저히 감소하였으며 혈중의 총콜레스테롤, 중성지방, glucose 의 함량이 유의적으로 감소하였고 또한, 간의 총콜레스테롤, 중성지방에서도 유의적으로 감소하였다. 열대사, β-oxidation과 관련한 lipolytic enzyme인 UCP-2와 CPT-1의 발현량은 유의적으로 증가하는 것을 확인할 수 있었다. 본 연구에서 꽃송이버섯이 3T3-L1세포의 지방분화를 억제하고 ob/ob 마우스에서 체중 및 지질대사를 개선 시키고 에너지 소비를 증가 시키므로 꽃송이버섯이 비만의 예방 및 치료 효과에 있어 새로운 후보물질이 될 것이라 사료된다.
Sprasis crispa (SC) is an edible mushroom that has high contents of β-(1,3)-D-Glucan, which has recently shown to exert various physiological effects on living organism. In this study, we examined the anti-obesity effects of hot water extract of S.crispa on adipogenesis in 3T3-L1 cell and lipid metabolism in obese mice. Using 3T3-L1 preadipocytes, cytotoxicity of SC were evaluated by MTT assay. SC treatment (0-1000㎍/ml) did not show toxicity in preadipocytes. Preadipocytes were treated withdifferentiation cocktail (MDI) in the absence or presence of various concentrations of SC (0, 200, 400 and 600㎍/ml). These samples were analyzed at day 2, 5 and 8 ofadipogenesis period. The results showed that SC had significantly inhibited lipid accumulation as compared with control as evidenced by ORO staining and TG assay, while glycerol release in SC treated cell was significantlyincreased. We also observed SC suppressed adipocyte differentiation by the reduction of protein expression of PPAR γ, C/EBP β. These results indicate that SC inhibits adipocyte development via down-regulated expression of adipogenic transcription factor. In order to investigate the in vivo effect, C57BL/6J (ob/ob) mice were fed with diet containing SC; SC [SC100 (100mg/bw kg/day) and SC300 (300mg/bw kg/day)] for 10 weeks. The body weight gain of SC groups was significantly lower than control (6.77% in SC100, 6.40% in SC300). The tissue weights in SC groups were also reduced. SC prevented fasting total cholesterol (34.68% in SC100, 37.95% in SC300), triglyceride (43.40% in SC100, 41.98% in SC300), and glucose (23.04% in SC100, 31.04% in SC300) level as compared to control. The hepatic triglyceride was lower in SC groups (51.9% in SC100, 60.2% in SC300) than control group and total cholesterol level was also lower in SC groups (34.68% in SC100, 37.95% in SC300) than control group. In addition SC increased the lipolytic expression of protein involved in lipid oxidation and thermogenesis (CPT-1, UCP-2) in liver. In conclusion, we may suggest the anti-obesity effects of SC in both 3T3-L1 cells and ob/ob mice.
Sprasis crispa (SC) is an edible mushroom that has high contents of β-(1,3)-D-Glucan, which has recently shown to exert various physiological effects on living organism. In this study, we examined the anti-obesity effects of hot water extract of S.crispa on adipogenesis in 3T3-L1 cell and lipid metabolism in obese mice. Using 3T3-L1 preadipocytes, cytotoxicity of SC were evaluated by MTT assay. SC treatment (0-1000㎍/ml) did not show toxicity in preadipocytes. Preadipocytes were treated withdifferentiation cocktail (MDI) in the absence or presence of various concentrations of SC (0, 200, 400 and 600㎍/ml). These samples were analyzed at day 2, 5 and 8 ofadipogenesis period. The results showed that SC had significantly inhibited lipid accumulation as compared with control as evidenced by ORO staining and TG assay, while glycerol release in SC treated cell was significantlyincreased. We also observed SC suppressed adipocyte differentiation by the reduction of protein expression of PPAR γ, C/EBP β. These results indicate that SC inhibits adipocyte development via down-regulated expression of adipogenic transcription factor. In order to investigate the in vivo effect, C57BL/6J (ob/ob) mice were fed with diet containing SC; SC [SC100 (100mg/bw kg/day) and SC300 (300mg/bw kg/day)] for 10 weeks. The body weight gain of SC groups was significantly lower than control (6.77% in SC100, 6.40% in SC300). The tissue weights in SC groups were also reduced. SC prevented fasting total cholesterol (34.68% in SC100, 37.95% in SC300), triglyceride (43.40% in SC100, 41.98% in SC300), and glucose (23.04% in SC100, 31.04% in SC300) level as compared to control. The hepatic triglyceride was lower in SC groups (51.9% in SC100, 60.2% in SC300) than control group and total cholesterol level was also lower in SC groups (34.68% in SC100, 37.95% in SC300) than control group. In addition SC increased the lipolytic expression of protein involved in lipid oxidation and thermogenesis (CPT-1, UCP-2) in liver. In conclusion, we may suggest the anti-obesity effects of SC in both 3T3-L1 cells and ob/ob mice.