학술논문
고리매개등온증폭법을 활용한 분자진단법과 현장진단으로의 적용에 관한 연구 / Loop-mediated isothermal amplification as a molecular diagnostics tool and the application for point-of-care testing
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
Korean
Abstract
고리매개등온증폭법 (Loop-mediated isothermal amplification, LAMP)은 등온에서 신속하게 핵산을 증폭하는 분자진단법의 한 방법으로, 기존의 qPCR (Real-time polymerase chain reaction)을 대체할 기술로 떠오르고 있다. 본 연구에서는 고리매개등온증폭법에 대해 고찰하고, 이를 활용하여 최근 의료 현장에서 선별 검사의 중요성이 제기되고 있는 선천 거대세포바이러스 (Cytomegalovirus, CMV) 감염과 의료 현장에서 집단 감염의 위험성이 존재하는 카바페넴 분해효소 생성 장내세균(carbapenemase-producing Enterobacteriaceae, CPE) 감염에 활용할 수 있는 선별검사를 개발하고, 나아가 의료 현장에서 현장진단 (point-of-care testing, POCT)로의 활용 가능성에 대해 고찰하였다.
Loop-mediated isothermal amplification (LAMP) amplifying targeted nucleic acid rapidly under isothermal condition is an upcoming molecular diagnosis technoloty in the replace for real-time polymerase chain reaction (qPCR). With a better understanding of LAMP, this study aims to develop LAMP assay for screening congenital cytomegalovirus (CMV), the importance of which is rising these days, and carbapenemase-producing Enterobacteriaceae screening, the infection of which could lead to massive infections in the medical field. Also, this study discusses the potential of the LAMP assay for point-of-care testing (POCT) in the medical field for the future.
Loop-mediated isothermal amplification (LAMP) amplifying targeted nucleic acid rapidly under isothermal condition is an upcoming molecular diagnosis technoloty in the replace for real-time polymerase chain reaction (qPCR). With a better understanding of LAMP, this study aims to develop LAMP assay for screening congenital cytomegalovirus (CMV), the importance of which is rising these days, and carbapenemase-producing Enterobacteriaceae screening, the infection of which could lead to massive infections in the medical field. Also, this study discusses the potential of the LAMP assay for point-of-care testing (POCT) in the medical field for the future.