학술논문
Studies for In situ Coating Method of Sodium Alginate-Hyaluronic Acid Hydrogels for Clinical Application
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
English
Abstract
천연 폴리사카라이드로 합성된 하이드로겔은 biomedical 분야에 다양하게 적용될 수 있는 유망한 재료이다. 이 연구에서 우리는 다른 비율(1:1~1:5)로 배합한 hyaluronic acid (HA)와 sodium alginate (AL)을 새로운 in situ 합성법을 통하여 이온결합과 공유결합에 의해 가교된 하이드로겔을 합성하였다. 합성된 하이드로겔의 화학적 구조는 FTIR을 통해 확인하였고 표면형태, 팽윤도, in vitro 생분해 역시 측정하였다. L929 mouse fibroblasts을 이용한 MTT 실험을 통해 하이드로겔의 무독성을 검증하였다. 의료등급의 polyurethane (PU, Pellethane??)과 함께 혈소판 흡착 실험을 수행하여 합성된 하이드로겔의 control-PU에 버금가는 혈액 적합성을 확인하였고, recombinant epidermal growth factor (rhEGF)을 하이드로겔에 주입하여 human L929 cell의 증식과 흡착의 향상을 확인하였다. 결론적으로 상기의 특징들을 종합해 볼 때 HAAL 하이드로겔은 향후 in situ 코팅을 통해 임상분야에 적용될 수 있을 것으로 예상된다.
Studies for In situ Coating Method of Sodium Alginate-Hyaluronic Acid Hydrogels for Clinical Application Hydrogel synthesized by natural polysaccharides seems to be promising materials for various biomedical applications. In this research, we report a novel in situ synthetic scheme for hydrogel derived from hyaluronic acid (HA) and sodium alginate (AL) in different compositions (from 1:1 to 1:5) with ionic and covalent crosslink. The chemical structures of synthesized hydrogles were characterized by FTIR spectroscopy. Surface morphologies, equilibrium swelling and in vitro biodegradation behaviors of hydrogels were also examined. The MTT assay demonstrated that the extract of hydrogels was non-toxic to L929 mouse fibroblasts. Compared with medical grade polyurethane (PU,Pellethane??), the hydrogels supported the equivalent blood compatibility as control-PU through platelet adhesion test. Followed by introducing the recombinant human epidermal growth factor (rhEGF) into hydrogels, they presented the ability to enhance the human L929 cell proliferation and adhesion behavior. In conclusion, these characteristics provide that the HAAL hydrogels may have potential usage in clinical applications through in situ coating method.
Studies for In situ Coating Method of Sodium Alginate-Hyaluronic Acid Hydrogels for Clinical Application Hydrogel synthesized by natural polysaccharides seems to be promising materials for various biomedical applications. In this research, we report a novel in situ synthetic scheme for hydrogel derived from hyaluronic acid (HA) and sodium alginate (AL) in different compositions (from 1:1 to 1:5) with ionic and covalent crosslink. The chemical structures of synthesized hydrogles were characterized by FTIR spectroscopy. Surface morphologies, equilibrium swelling and in vitro biodegradation behaviors of hydrogels were also examined. The MTT assay demonstrated that the extract of hydrogels was non-toxic to L929 mouse fibroblasts. Compared with medical grade polyurethane (PU,Pellethane??), the hydrogels supported the equivalent blood compatibility as control-PU through platelet adhesion test. Followed by introducing the recombinant human epidermal growth factor (rhEGF) into hydrogels, they presented the ability to enhance the human L929 cell proliferation and adhesion behavior. In conclusion, these characteristics provide that the HAAL hydrogels may have potential usage in clinical applications through in situ coating method.