학술논문
Pharmacological properties of Lindera glauca / 감태나무의 약리 활성
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
English
Abstract
국문 초록감태나무의 약리 활성우석대학교 약학대학원한약학과 한약학 전공김 종 수(지도교수 : 전 훈 Ph.D.)감태나무 (Lindera glauca)는 관절염, 근육통, 타박상 등 많은 질환의 치료에 다양하게 사용되어져 왔다. 본 연구에서는 감태나무의 ethyl acetate 분획이 항산화, 항염증 및 진통에 미치는 약리작용에 대해 조사하였다. 수종의 자유기에 대한 항산화 실험 결과 감태나무가 농도의존적인 강한 자유기 소거능 및 환원력을 가지고 있는 것으로 확인되었다. 또한 감태나무는 쥐의 복강 대식세포에서 IFN-γ와 LPS의 자극에 의해 생성되는 NO를 효과적으로 억제하였으며 이는 iNOS enzyme의 활성 저해와 함께 염증관련 매개효소인 iNOS와 COX-2의 단백질발현 감소에 기인하였다. 이러한 염증매개물질의 억제효과는 전사인자인 NF-κB의 핵 내이동 저해에서 비롯된 것임이 확인되었다. 또한 감태나무는 tyrpsin으로 유도된 부종 역시 현저히 감소시켰다. 다양한 진통실험에서 감태나무는 중추성 및 말초성 통증에 대해 모두 유의성 있는 진통효과를 보여주었으며 naloxone의 전처리에 의해 진통작용이 감소하지 않은 것으로 보아 감태나무의 진통작용은 opioid receptor 이외의 기전을 통해 나타남을 확인할 수 있었다. 이러한 결과로 미루어보아 감태나무는 항산화, 항염증 및 진통 작용을 가지고 있는 것을 알 수 있었으며 이와 관련한 여러 질환에 효과적으로 활용될 수 있을 것으로 사료된다.
AbstractPharmacological activities of Lindera glaucaKim, Jong SooDept. of Oriental PharmacyCollege of PharmacyGraduate School of Woosuk University(Directed by Prof. Jeon, Hoon, Ph.D.)Lindera glauca (Lauraceae) has been widely used as a traditional medicine for the treatment of lots of diseases like arthritis, myalgia, contusion. In the present study, the radical scavenging tests of the ethylacetate fraction of L. glauca (ELG) against DPPH radical, superoxide anion, nitric oxide and ABTS radical were perpormed. The results showed that ELG had potent antioxidant capacity and reducing power compared to positive control such as ascorbic acid. The author also evaluated ELG's anti-iflammatory properties using IFN-γ and LPS-activated murine peritoneal macrophage model. ELG suppressed NO production and iNOS expression via down-regulation of NF-κB activation. It also attenuated the expression of COX-2. ELG also significantly suppressed trypsin-induce paw edema. Moreover, ELG demonstrated strong and dose-dependent anti-nociceptive activities on various experimental pain models including thermal nociception and chemical nociception, compared to Tramadol and Indomethacin, a reference drug. In combination test using naloxone, analgesic activity of ELG was not changed indicating the antinociception of ELG was not due to acting as an opioid receptor agonist. The present results indicate that ELG may possibly used as valuable radical scavenging, anti-inflammatory and anti-nociceptive agent for the treatment of various diseases.
AbstractPharmacological activities of Lindera glaucaKim, Jong SooDept. of Oriental PharmacyCollege of PharmacyGraduate School of Woosuk University(Directed by Prof. Jeon, Hoon, Ph.D.)Lindera glauca (Lauraceae) has been widely used as a traditional medicine for the treatment of lots of diseases like arthritis, myalgia, contusion. In the present study, the radical scavenging tests of the ethylacetate fraction of L. glauca (ELG) against DPPH radical, superoxide anion, nitric oxide and ABTS radical were perpormed. The results showed that ELG had potent antioxidant capacity and reducing power compared to positive control such as ascorbic acid. The author also evaluated ELG's anti-iflammatory properties using IFN-γ and LPS-activated murine peritoneal macrophage model. ELG suppressed NO production and iNOS expression via down-regulation of NF-κB activation. It also attenuated the expression of COX-2. ELG also significantly suppressed trypsin-induce paw edema. Moreover, ELG demonstrated strong and dose-dependent anti-nociceptive activities on various experimental pain models including thermal nociception and chemical nociception, compared to Tramadol and Indomethacin, a reference drug. In combination test using naloxone, analgesic activity of ELG was not changed indicating the antinociception of ELG was not due to acting as an opioid receptor agonist. The present results indicate that ELG may possibly used as valuable radical scavenging, anti-inflammatory and anti-nociceptive agent for the treatment of various diseases.