부산대학교 도서관

Objectives : The aim of this study was to evaluate the distribution of the bacteria associated with dental caries and periodontal disease in saliva and plaque of the children and adolescents. The correlation between the indices related to caries were compared and analyzed according to age.Methods : Subjects were total of 90 and they were consisted by thirty 2nd graders in Elementary school, thirty 2nd graders in High school and thirty adults in late 20s. Saliva and plaque were collected from subjects and DMFT index and Significant Caries(SiC) index were assessed. The samples were cultivated on Dentocult-SMTM and MS agar medium to measure the colony density(CFU/ml) of Streptococcus mutans (S. mutans). In additions, the presence of S. mutans and other periodontal pathogens (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia) were detected using PCR. The salivary levels of S. mutans were measured using 4 types of monoclonal antibodies(ckAg I/II, ckBN, ckCN, ckDN) which were developed to target Ag I/II and Glucosyltransferase(Gtf B, Gtf C, Gtf D). Furthermore, the correlations between the dependent variables were compared.Results : S. mutans detected in total 59 saliva samples; 13 (group 1), 22 (group 2) and 24 (group 3). S. mutans detected in total 42 plaque samples; 19 (group 1), 14 (group 2) and 9 (group 3). A. actinomycetemcomitans was detected the most in group 2 and T. forsythia, F. nucleatum were detected the most in group 1. Salivary S. mutans levels were higher in female than male in each group without significant difference (p > 0.05) while salivary S. mutans levels in female and male of group 2 were both significantly higher than other groups (males; p < 0.05, females; p < 0.01). There were no statistically significant relationship between DMFT index and salivary S. mutans levels. The detected result of S. mutans and other periodontal pathogens using PCR showed that such bacteria were significantly better detected in saliva than supragingival plaque. Generally, there was positive correlation between DMFT index and 4 types of monoclonal antibodies.Conclusions : S. mutans and other periodontal pathogens were detected more in adolescents than children. Such results suggest that oral hygiene needs to emphasized more in adolescents and they may be utilized for the early prevention and the related research of dental caries and periodontal disease.