학술논문
함치성 낭종 유래 성체 줄기세포의 osteogenic potential / EValuation of osteogenic potential of mesenchymal stem cells from dentigerous cyst
Document Type
Dissertation/ Thesis
Author
최찬 / Choi, Chan
Source
Subject
Language
Korean
Abstract
연구 목적: 본 연구는 함치성 낭종 유래 세포의 골모세포로의 분화 및 골형성능을 밝히기 위하여 골형성 유전자의 발현을 분석하여 향후 골재생에 사용이 가능한지 분석하고자 하였다. 연구 재료 및 방법: 치수 줄기세포와 함치성 낭종 줄기세포를 하악 매복 제3 대구치와 이에 연관되어 발생된 함치성 낭종에서 추출하고 Flow cytometric analysis와 Real-time PCR analysis를 시행하였다. 연구 결과: 함치성 낭종 줄기세포는 Alkaline phosphatse(ALP) 염색과 von Kossa 염색을 통하여 골모세포로 분화되었고 Real-time PCR 결과 osteogenic potential을 지니고 있었다. 결론: 함치성 낭종 줄기세포는 골재생에 사용할 수 있는 줄기세포원으로 생각된다.
Aim of the study: The aim of this study was to analyze the gene expression of dentigerous cyst stem cells(DCSCs) to highlight the osteogenic potential and advantages of their use in bone regeneration. Materials & methods: Dental pulp stem cells(DPSCs) and Dentigerous cyst stem cells(DCSCs) were isolated from dentigerous cyst and associated impacted lower third molars, and flow cytometric analysis was done. Cells were cultured, induced to differentiated to osteoblasts, and gene expression study was done using Real-time PCR analysis. Results: DCSCs differentiation was confirmed in Alkaline phosphatse(ALP) staining and von Kossa staining, The results of Real-time PCR analysis suggest that DCSCs have osteogenic potential. Conclusion: DCSCs could be a useful alternative source of mesenchymal stem cells for bone regeneration.
Aim of the study: The aim of this study was to analyze the gene expression of dentigerous cyst stem cells(DCSCs) to highlight the osteogenic potential and advantages of their use in bone regeneration. Materials & methods: Dental pulp stem cells(DPSCs) and Dentigerous cyst stem cells(DCSCs) were isolated from dentigerous cyst and associated impacted lower third molars, and flow cytometric analysis was done. Cells were cultured, induced to differentiated to osteoblasts, and gene expression study was done using Real-time PCR analysis. Results: DCSCs differentiation was confirmed in Alkaline phosphatse(ALP) staining and von Kossa staining, The results of Real-time PCR analysis suggest that DCSCs have osteogenic potential. Conclusion: DCSCs could be a useful alternative source of mesenchymal stem cells for bone regeneration.