부산대학교 도서관

Succinic acid is widely applied to chemical,pharmaceutical, food, and agricultural industries. With therapid development of these industries, a great demand ofsuccinic acid is required. The acid-tolerance and succinicacid production of Actinobacillus succinogenes strain wereimproved by using genome shuffling. Results showed thatone modified strain AS-F32, with the best acid resistanceand the highest succinic acid production, was obtained after3 cycles of genome shuffling. The minimum growth pH ofAS-F32 was 3.5, and the acid production and cell dryweight were 5.1 and 4.8 g/L in flask, improved 2.6 and1.85 times over the start strain As-R2. Furthermore, thesuccinic acid yield of As-32 was 31.2 g/L and the dry cellweight was increased 44.4% by maintaining pH 4.8 with7.0 M NH4OH in 5 L bioreactor, increased 1.1 times thanthe original strain As-R2.