학술논문
Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration
Document Type
Article
Author
Wu Jiang; Fu Liwei(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, Institute of Orthopedics, Chinese PLA General Hospital); Yan Zineng; Yang Yu; Yin Han(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Li Pinxue(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Key Laboratory of Musculoskeletal Trauma); Yuan Xun; Ding Zhengang; Kang Teng; Tian Zhuang(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Liao Zhiyao(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Tian Guangzhao(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Ning Chao(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Li Yuguo(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Sui Xiang(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Chen Mingxue(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Liu Shuyun(Beijing Key Laboratory of Regenerative Medicine in Orthopedics; Guo Quanyi
Source
생체재료학회지, 27(00), pp.490-507 Mar, 2023
Subject
Language
English
ISSN
2055-7124
1226-4601
1226-4601
Abstract
In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more endogenous stem cells from the joint cavity to the defect area to promote cartilage regeneration.In this study, we used ECM alone as a bioink in low-temperature deposition manufacturing (LDM) 3D printing and then successfully fabricated a hierarchical porous ECM scaffold incorporating GDF-5.Comparative in vitro experiments showed that the 7% ECM scaffolds had the best biocompatibility. After the addition of GDF-5 protein, the ECM scaffolds significantly improved bone marrow mesenchymal stem cell (BMSC) migration and chondrogenic differentiation. Most importantly, the in vivo results showed that the ECM/GDF-5 scaffold significantly enhanced in situ cartilage repair.In conclusion, this study reports the construction of a new scaffold based on the concept of in situ regeneration, and we believe that our findings will provide a new treatment strategy for AC defect repair.