부산대학교 도서관

Echinacoside (ECH) is a prominent naturally occurring bioactive compound with effects of alleviatingmyocardial damage. We aimed to explore the beneficial effects of ECH against sepsis-induced myocardial damage andelucidate the potential mechanism. Echocardiography and Masson staining demonstrated that ECH alleviates cardiac functionand fibrosis in the cecal ligation and puncture (CLP) model. Transcriptome profiling and network pharmacology analysisshowed that there are 51 overlapping targets between sepsis-induced myocardial damage and ECH. Subsequently, chemicalcarcinogenesis-reactive oxygen species (ROS) were enriched in multiple targets. Wherein, SOD2 may be the potential targetof ECH on sepsis-induced myocardial damage. Polymerase chain reaction results showed that ECH administration couldmarkedly increase the expression of SOD2 and reduce the release of ROS. Combined with injecting the inhibitor of SOD2, thebeneficial effect of ECH on mortality, cardiac function, and fibrosis was eliminated, and release of ROS was increased afterinhibiting SOD2. ECH significantly alleviated myocardial damage in septic mice, and the therapeutic mechanism of ECH isachieved by upregulating SOD2 which decreased the release of ROS.