부산대학교 도서관

Background Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determiningthe physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1in hair follicle development have so far not been reported. Objective This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development. Methods Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors andinterference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues,and the effects on other hair follicle development-related genes. Results The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localizedmostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, itwas found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally duringhair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2,KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P < 0.05), the converse after the corresponding useof si-RNA interference. Conclusions Krtap11-1 exerts a promoting effect. The results provide novel insight into the relationship between hair follicledevelopment and Krtap11-1 gene expression.