부산대학교 도서관

Astragalin, a bioactive component of medicinalplants such as Rosa agrestis, has anti-inflammatory andantioxidant features. Induction of heme oxygenase (HO)-1is an effective strategy to reduce excessive generated oxidantsduring the pathogenesis of acute lung injury (ALI). The aim of the present study is to investigate that whetherthe anti-inflammatory and antioxidant features of astragalinis HO-1 dependent in lipopolysaccharide (LPS)-inducedALI. Sprague–Dawley rats were used in animal study. Intratracheal LPS was performed to induce experimentalALI model. Astragalin was administrated 1 h after LPSchallenge. Human lung epithelial cells were used in cellstudy. Samples from rats were harvested at 24 h post LPSchallenge. Astragalin treatment inhibited LPS-inducedinflammatory cells infiltration in the lung and pulmonaryedema. Astragalin treatment markedly enhanced theactivity of HO-1 compared with vehicle-treated group at24 h post LPS challenge. Levels of lipid hydroperoxide, amarker for oxidative stress, were decreased in astragalintreatedanimals compared with vehicle-treated group. However, the protective effect of astragalin on LPS-inducedALI was abolished in an inhibitor of HO-1-treatedanimals. Moreover, the astragalin-induced the upregulationof HO-1 in human lung epithelial cells was inhibited whennuclear factor erythroid-2-related factor 2 (Nrf2) wassilenced by small interfering RNA. Astragalin reducesLPS-induced ALI via activation of Nrf2/HO-1 pathway.