부산대학교 도서관

Diacylglycerol (DAG) was prepared viaglycerolysis of palm oil catalyzed by Lecitase Ultra (LU),a novel phospholipase from the fusion of lipase genes fromThermomyces lanuginose and phospholipase genes fromFusarium oxysporum. Glycerolysis was performed in asolvent-free system. The optimized reaction conditionswere: a glycerol/palm oil mole ratio of 7.5:1, initialsubstrate water content of 5%, substrate enzyme load of2%, reaction temperature of 40oC, and reaction time of 8 h. In a scale-up reaction, a DAG content of 59.5% in the lipidlayer was achieved. Through a two-step molecular distillation,the composition of the target product was 88.1% DAG,2.8% TAG, 9.0% MAG, and 0.1% FFA. The fatty acidcomposition of the DAG oil, determined using GC-MS,was enriched compared with the original palm oil.