학술논문
A novel wheat cysteine-rich receptor-like kinase gene CRK41 is involved in the regulation of seed germination under osmotic stress in Arabidopsis thaliana
Document Type
Article
Author
Source
Journal of Plant Biology(한국식물학회지), 60(6), pp.571-581 Dec, 2017
Subject
Language
English
ISSN
1226-9239
Abstract
The bread wheat gene TaCRK41 encodes acysteine-rich receptor kinase. It is down-regulated by variousabiotic stresses and by exposure to abscisic acid. A transientexpression experiment in Arabidopsis thaliana protoplastsinvolving a fusion between the sequence encoding greenfluorescence protein and the TaCRK41 allele from either thesalinity tolerant bread wheat cultivar Shanrong No. 3 (SR3)or its salinity sensitive progenitor cultivar Jinan 177 (JN177)showed that the TaCRK41 product is deposited in thecytoplasm. Recombinant TaCRK41 originating from bothSR3 and JN177 displayed no kinase activity in vitro. Theconstitutive expression of TaCRK41 in A. thaliana resultedin a marked reduction in the plants' sensitivity to bothexogenous abscisic acid and to salinity during germination. The level of ABA-induced transcription of ABI3, ABI5 andthe ABI5-controlled genes EM1 and EM6 was significantlyreduced in plants subjected to stress. The data support theidea that TaCRK41 is involved in the regulation of ABAdependentgermination under conditions of osmotic stress.