부산대학교 도서관

Background and aim: Panax ginseng, a key herbal medicine of replenishing Qi and tonifying Spleen, iswidely used in the treatment of gastrointestinal diseases in East Asia. In this study, we aim to investigatethe potential effects and mechanisms of polysaccharides from P. ginseng (PGP) on intestinal mucosalrestitution which is one of the crucial repair modalities during the recovery of mucosal injury controlledby the Ca2þ signaling. Methods: Rat model of intestinal mucosal injury was induced by indomethacin. The fractional cellmigration was carried out by immunohistochemistry staining with BrdU. The morphological observationson intestinal mucosal injury were also performed. Intestinal epithelial cell (IEC-6) migration in vitrowas conducted by scratch method. Western-blot was adopted to determine the expressions of PLC-g1,Rac1, TRPC1, RhoA and Cav-1. Immunoprecipitationwas used to evaluate the levels of Rac1/PLC-g1, RhoA/TRPC1 and Cav-1/TRPC1. Results: The results showed that PGP effectively reduced the assessment of intestinal mucosal injury,reversed the inhibition of epithelial cell migration induced by Indomethacin, and increased the level ofCa2þ in intestinal mucosa in vivo. Moreover, PGP dramatically promoted IEC-6 cell migration, theexpression of Ca2þ regulators (PLC-g1, Rac1, TRPC1, Cav-1 and RhoA) as well as protein complexes (Rac1/PLC-g1, Cav-1/TRPC1 and RhoA/TRPC1) in vitro. Conclusion: PGP increases the Ca2þ content in intestinal mucosa partly through controlling the regulatorsof Ca2þ mobilization, subsequently promotes intestinal epithelial cell migration, and then preventsintestinal mucosal injury induced by indomethacin.