부산대학교 도서관

Baculoviruses serve as efficient viral vectors for gene delivery into vertebrate and invertebrate cells. The identificationand characterization of the functional promoters in different baculovirus-infected hosts are essentialfor the efficient gene expression. To establish a baculovirus-mediated gene transfer system in the silkworm,Bombyxmori, we investigated the activities of silkworm-derived TCTP, ACTIN3, and HSC70-4 promoters deliveredby AcNPV or BmNPV in various tissues of silkworm. In many of the tested silkworm tissues, the BmHSC70-4promoter exhibited a higher transcription activity than those of BmTCTP or BmACTIN3 promoters when deliveredby AcNPV, which is reported to be incapable of replicating in silkworms. In contrast, the BmACTIN3 promoter wasfound to be the strongest promoterswhen delivered by BmNPV. The present results indicate that the BmHSC70-4promoter is potentially useful for the stable gene expression by the non-replicating AcNPV vector for gene functionanalysis in the silkworm.