부산대학교 도서관

There is little evidence regarding the roles oflong noncoding RNAs (lncRNAs) in inflammation caused byasthma. In this study, we successfully generated an asthmamouse model that was induced by ovalbumin (OVA). Theeffects of dexamethasone (Dex) treatment on lung tissuewere investigated using pathological and biochemicalmethods, including Diff-Quik staining, enzyme-linkedimmunosorbent assay (ELISA), hematoxylin–eosin (H&E)staining, and western blotting (WB). The inflammation waseffectively relieved with Dex treatment. High-throughputsequencing revealed that a total of 1490 lncRNAs were detected in lung tissue samples. Differential expressionanalysis revealed that the Dex group had 20 upregulatedand 15 downregulated lncRNAs compared with those inthe Model group. Moreover, nine differentially expressedand inflammation-related lncRNAs were verified by quantitativereal-time reverse transcription polymerase chainreaction (qRT-PCR). Furthermore, the regulation networksof these nine lncRNAs, their potential binding microRNA(miRNAs), and the putative target genes showed that theselncRNAs play important roles in the nuclear factor kappalight-chain-enhancer of activated B cells (NF-κB) signalingpathway. We further identified the expression levels of threepotential binding miRNAs by qRT-PCR. The results of thisstudy contribute to a better understanding of the functionsof lncRNAs in inflammation caused by asthma.