부산대학교 도서관

The efficacy of magnetic nanoparticles (MNPs) for biomedical applications depends on the specic targetingcapacity, blood circulation time and magnetic susceptibility. Functionalized chitosan-coated Fe3O4 nanoparticles(CS-coated Fe3O4 NPs) were synthesized by a non-solvent-aided coacervation procedure followed by a chemicalcrosslinking procedure. The surfaces of CS-coated Fe3O4 NPs were successfully functionalized with folate-poly(ethyleneglycol)-COOH (FA-PEG) to obtain novel FA-PEG-CS-coated Fe3O4 NPs endowed with long blood circulationand specic targeting capacity. The as-synthesized NPs were characterized by dynamic light scattering, transmissionelectron microscope, X-ray diffraction, thermal gravimetric analysis, vibration sample magnetometer, Fourier transforminfrared spectroscopy, and confocal laser scanning microscopy. As a result, the novel FA-PEG-CS-coated Fe3O4 NPsshowed excellent biocompatibility, magnetic properties, good dispersibility, and proper hydrodynamic sizes in an aqueousmedium. The specific targeting capacity of the as-synthesized NPs to cancer cells was also investigated. It wasobserved that the uptake of the FA-PEG-CS-coated Fe3O4 NPs by HeLa cells was significantly enhanced comparedto the CS-coated Fe3O4 NPs and mPEG-CS-coated Fe3O4 NPs. These results clearly indicate that our novel FA-PEGCS-coated Fe3O4 NPs with remarkable specific targeting capacity, long blood circulation, and superparamagnetismhold great promise for biomedical applications, including targeted drug delivery and hyperthermia therapy.