학술논문
홍삼 Ginsenoside의 Cytochrome P450 저해 활성 평가
In vitro Assessment of Cytochrome P450 Inhibition by Red Ginseng Ginsenosides
In vitro Assessment of Cytochrome P450 Inhibition by Red Ginseng Ginsenosides
Document Type
Article
Source
약 학 회 지, 59(2), pp.49-54 Apr, 2015
Subject
Language
한국어
ISSN
2383-9457
0377-9556
0377-9556
Abstract
In the present study we evaluated comparative herb-drug interaction potential of red ginseng total powder, ginsenosideRg1, and Rb1 by inhibition of CYP isoforms including CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6,CYP2E1 and CYP3A4 using pooled human liver microsomes (HLMs). As measured by liquid chromatography-electrosprayionization tandem mass spectrometry, red ginseng total powder inhibited significantly activities of CYP1A2, CYP2A6,CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and testosterone 6-beta hydroxylation by CYP3A4, but the IC50 valueswere higher than 556 μg/ml. Activities of CYP2B6, CYP2C9, CYP2D6 and CYP3A4 were inhibited by ginsenoside Rb1. Also,activities of CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6 and testosterone 6-beta hydroxylation by CYP3A4were inhibited by ginsenoside Rg1. The IC50 values of ginsenoside Rb1 and Rg1 were higher than 200 μg/ml. Based on IC50values against CYP isoforms, ginsenosides-drug interactions by CYP inhibition may be very low in clinical situations.