부산대학교 도서관

In this study, thrombin-conjugated PLGA (THR-PLGA) electrospun meshes were prepared throughreagent free surface modification based on Schiff base reaction between amine groups in thrombin andcarbonyls on the surface of PLGA meshes, and their feasibilities on the improvement of hemostasis wereevaluated. In vitro studies were performed with chromogenic substrate and transglutaminase activityassays to evaluate the thrombin activity and FXIIIa activity of the THR-PLGA samples, respectively. Arat tail injury model was employed for measuring hemostatic time of the samples. The results showedthat the hemostatic efficacy of the samples increased with increase in the content of THR on the surfaceof the samples. In addition, partial splenectomy was carried out in rat model to investigate the potentialmanagement of hemorrhage of THR-PLGA, as compared to Vicryl and TachoSil. The results showed thatthe inflammatory response was significantly reduced compared to Vicryl and TachoSil, and a densefibrous layer was produced to protect the inside from laceration at 2 weeks of treatment. Moreover,neo pulps were appeared right below the fibrous capsule. These findings suggest that THR-PLGA samplesprepared through the reagent free immobilization method deserves further advanced investigations forfuture clinical applications.