부산대학교 도서관

In this study, dikaryotic strains of Lentinula edodes were generated by mono-monohybridization using mating type analysis and their fruiting body characteristics wereinvestigated. Approximately 100 monokaryotic strains were isolated from the basidiosporesof Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho usingprotoplast isolation and regeneration. Their mating types were evaluated and a total of 60dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced andtheir characteristics were examined after cultivation on sawdust media. The results indicatedthat the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruitingbodies with abnormal pileus and gill structures. The weight and size of the fruiting bodiesdiffered depending on the strains. Overall, further studies are needed for predicting thecharacteristics of hybridized strains based on their parental strains.