부산대학교 도서관

In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and thereconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increasedthe expression of cyclin D1 and decreased the expression of p27. Fucoidan was used to reconstructSE. Immunohistochemical staining showed that the addition of fucoidan to dermal equivalents increasedexpression of proliferating cell nuclear antigen (PCNA) and p63. In addition, expression ofα6-integrin was significantly increased by fucoidan, whereas expression of β1-integrin, type 1 collagen,elastin, fibronectin did not markedly change. These results suggest that fucoidan has positive effectson epidermal reconstruction and will therefore be beneficial in the reconstruction of SE.