학술논문
Observation of intracellular localization of a neurite outgrowth factor, Dns-Leu-Leu-Leu-H by using fluorescence microscopy / 蛍光顕微鏡による神経突起伸長因子Dns-Leu-Leu-Leu-Hの細胞内局在観察
Document Type
Journal Article
Author
Source
東京保健科学学会誌 / The Journal of Tokyo Academy of Health Sciences. 2002, 5(3):157
Subject
Language
Japanese
ISSN
1344-3844
2433-149X
2433-149X
Abstract
An exogenous drug, benzyloxycarbonyl-Leucyl-Leucyl-Leucinal (Z-LLL-H) stimulates neurite outgrowth of PC12 cells. In this study, we observed the localization of a fluorescent peptidylaldehyde (Dns-LLL-H) in the cells by using fluorescence microscopy. Dns-LLL-H induced neurite outgrowth at the concentration of 10nM as well as Z-LLL-H. It was taken up within 10min after treatment and was concentrated spottily at the cell bodies, neurites and growth cones, but not at the nuclei and filopodia. These results suggest that Dns-LLL-H is localized at organelles of PC12 cells such as mitochondria and stimulates neurite outgrowth.