학술논문
The Role of ASF1 and CAF-1 in Morphogenesis of Arabidopsis / シロイヌナズナ形態形成におけるヌクレオソーム形成関連遺伝子群の役割
Document Type
Journal Article
Author
Daisuke Shibata; Hidetaka Kaya; Hiroaki Hayashi; Jong Hwan Lee; Joung Hee BAEK; Kazuo Shinozaki; Keiichi Shibhara; Masatomo Kobayashi; Satoshi Tabata; Takashi Araki; Tomoaki Seki; Tomohiko Kato; Tomohiro Kubo; Yumiko Shirano; 久保 知大; 加藤 友彦; 小林 正智; 李 鐘煥; 林 浩昭; 柴原 慶一; 柴田 大輔; 田畑 哲之; 白 靜熹; 白野 由美子; 篠崎 一雄; 荒木 崇; 賀屋 秀隆; 関 原明
Source
Plant and Cell Physiology Supplement. 2003, :46
Subject
Language
Japanese
Abstract
CAF-1, a histone binding complex, assembles nucleosome preferentially onto newly replicated DNA in SV40 DNA replication in vitro system. ASF1, another histone binding protein, is known to facilitate CAF-1-dependent nucleosome assembly in vitro. Many other observations suggested that CAF-1 and ASF1 play a critical role in nucleosome assembly during DNA replication also in proliferating cells. However, physiological implications of those factors in multicellular organisms remain to be unknown. In order to analyze physiological role of ASF1, we isolated T-DNA inserted mutants of ASF1a and ASF1b, two functional homologues of ASF1 in Arabidopsis. Although asf1a and asf1b mutants did not show any significant morphological phenotypes so far now, asf1a;asf1b double mutants showed distinct phenotypes including decreased the number of lateral roots and defect in the cell files of RAM. Our observations implied that ASF1