부산대학교 도서관

Feces from silkworm larvae, Bombyx mori, infected with Bombyx densonucleosis virus type-1 (DNV-1) and type-2 (DNV-2) contain viruses particles. A polymerase chain reaction (PCR) was used to detect DNV infected silkworm larvae without killing. Newlyecdysed 4th instar larvae of susceptible and nonsusceptible (completely resistant) strains were fed DNVs 24 hours prior to the collection of feces. Feces were collected daily and DNA extracted from a single specimen of fecal matter was used as a template for PCR. In the susceptible strains, DNVs were detected in all fecal specimens beginning the day after inoculation to the day of viral death. In the nonsusceptible strains, DNVs were detected only in fecal matter collected the day after inoculation and were not detected on and after the 2nd day. Based on these studies, we were able to diagnose rapidly both DNV-1 and DNV-2 infection using PCR to detect DNVs in fecal matter.