부산대학교 도서관

Carp Cyprinus carpio (about 130g in body weight) were exposedto 2, 10 and 42 ppb Cd (CdCl2⋅ 21/2H2O) for 15, 29, 64 and 120 days. The water quality of control aquarium were as follows: water temperature, 21.3-25.2°C; dissolved oxygen concentration, 6.0-7.6mg/l; pH, 7.3-7, 5; water hardness, 60mg CaCO3/l.The fish exposed to 42 ppb Cd consumed less food and grew less than control, 2 and 10 ppb.Most of them died after 29 days. Fish affected by Cd were injured by pecking of other fish. There was little effect of Cd on red blood cell count, hemoglobin concentration, hematoclit value and serum levels or activities of Na, K, Mg, urea-N, triglyeeride, total cholesterol, LDH, AI-P and amylase. On the other hand, serum levels or activities of Ca, Cl, total protein, albumin, globulin, glucose and LAP inclined to decrease, while serum activities of GOT, GPT and LDH inclined to increase. As histopathological changes, swelling of gill epithelial cells, atrophy of hepatic and pancreatic cells, hyperplasia of hematopoietic cells, expansion of renal tubule and atrophy ofrenal epithelial cells in kidney were observed. And Cd-exposed fish showed scoliosis mainly in 12-15th vertebra and damage of neural spine.These hemochemical changes seem to be due to the changes of gill epithelial permeability andthe damage of hepatopancreas and renal tubule reabsorption. Activation of osteoclast and scoliosis which seem to be due to decrease of serum Ca concentration were also observed.