학술논문
INACTIVATION OF ENDOTHELIUM-DEPENDENT RELAXING FACTOR BY LIPOSOME-ENCAPSU-LATED OR CELL-FREE HEMOGLOBIN / リン脂質薄膜ヘモグロビン小胞体と単独ヘモグロビンによる血管内皮由来弛緩因子不活化の比較
Document Type
Journal Article
Author
E. TSUCHIDA; H. AKAMA; K. NAKAI; N. MATSUDA; S. SEKIGUCHI; S. TOKUYAMA; T. OHTA; Y. KAWAKAMI; Y. NAKAZATO; 中里 幸和; 仲井 邦彦; 土田 英俊; 太田 利男; 川上 吉久; 徳山 悟; 松田 典子; 赤間 和博; 関口 定美
Source
人工臓器 / Jinko Zoki. 1994, 23(3):854
Subject
Language
Japanese
ISSN
0300-0818
1883-6097
1883-6097
Abstract
The inhibitory effects of cellular and acellular hemoglobin (Hb) solutions on endothelium-dependent vasorelaxation were investigated in rabbit aortic strips. The tissues were precontracted with phenylephrine, after which acetylcholine (ACh) was added to induce a steady-state relaxation. All Hb solutions cumulatively reversed ACh-induced relaxation; liposome-encapsulated Hb and washed human red cells as cellular Hb reached a maximal vasoconstriction at 1mg/ml, while 2, 3-diphosphoglycerate depleted Hb and pyridoxylated Hb as acellular Hb reached a plateau at 10μg/ml. On the other hand, increasing in oxygen affinity by pyridoxylation had little effect on this. These findings suggest that the cellularity is very important factor influencing EDRF inhibition, and that acellular Hbs are more potent vasoconstrictor than cellular Hbs by a factor of about one hundred.