학술논문
Notch-Hes経路阻害を指標とする神経幹細胞分化活性化剤の探索 / Search for activator of neural stem cells differentiation based on inhibition of Notch-Hes signaling pathway
Document Type
Journal Article
Author
Source
Symposium on the Chemistry of Natural Products, symposium papers. 2015, :1
Subject
Language
Japanese
ISSN
2433-1856
Abstract
Five cardenolide glycosides (13-17) were isolated from Nerium indicum (leaves). 13inhibited the transcriptional activity of NICD with IC50 values of 0.12 mM. Western blot analysis of 13showed decrease in Hes1 and Hes5 protein expression. Uscharin (18) and calotropin (19) were also found as Notch signaling inhibitors. Next, we examined the effect of 13, 18 and 19on NSCs differentiation. These compounds accelerated differentiation of mouse NSCs (MEB5) into neurons and glial cells.