학술논문
The regulatory effects of sleep-related neuropeptide on the upper airway muscle contraction / セボフルラン麻酔下の睡眠関連神経ペプチドの上気道開大筋への効果
Document Type
Journal Article
Author
Source
Proceedings for Annual Meeting of The Japanese Pharmacological Society. 2023, :3-2
Subject
Language
Japanese
ISSN
2435-4953
Abstract
Spontaneously breathing C57BL/6J mice, anesthetized with sevoflurane, underwent measurement of heart rate and GG activity. Intracerebroventricular administration of orexin or peripherally administered orexin type 2 selective receptor agonist resulted in increased GG muscle contraction. To identify the brain nucleus responsible for gasping, we analyzed activated neurons immunohistochemically using an anti-Fos antibody. Mice exhibiting gasping (2 MAC sevoflurane) showed a significantly decreased number of choline acetyltransferase/Fos double-positive cells in the pedunculopontine tegmental nucleus and laterodorsal tegmental nucleus compared to control mice {0.7 Minimum Alveolar Concentration (MAC) sevoflurane}, suggesting inhibition of cholinergic-mediated depression of GG muscle activity. Additionally, we conducted a comprehensive analysis of differentially expressed genes involved in the development and maintenance of gasping breaths, which may offer new therapeutic targets for AHS.