학술논문
Conditions of examination of gastric cancer by HER2 FISH method using a commercially available kit—Comparison of signal visibility at various treatment times— / 市販キットを用いた胃癌HER2 FISH法の条件検討―各処理時間におけるシグナル視認性の比較―
Document Type
Journal Article
Author
Source
医学検査 / Japanese Journal of Medical Technology. 2015, 64(1):72
Subject
Language
Japanese
ISSN
0915-8669
2188-5346
2188-5346
Abstract
Fluorescence in situ hybridization (FISH) methods are an important technique for pathological diagnosis and the estimation of the efficacy of molecular targeted drug therapy. The HISTRA HER2 FISH kit (JOKOH) is a commercially available system by which FISH methods can be performed in a short time. We examined the conditions of heat treatment and enzyme treatment time in FISH methods using the HISTRA HER2 FISH kit in gastric cancer. Ten cases of gastric cancer were diagnosed in Mie University Hospital. The signal visibility was improved under the study conditions, namely, 40 min of heat treatment and 10 min of enzyme treatment, and 60 min of heat treatment and 10 min of enzyme treatment. Extension of the heat treatment time was considered to improve signal visibility.