부산대학교 도서관

The anticonvulsant effect of ketogenic diet was evaluated by using various expermental seizure models, and the concentrations of brain amino acids and monoamines in young mice under ketogenic diet were investigated by high performance liquid chromatography.Four-week-old male mice (ddY strain) were fed on MCT powdered milk admixed with cellulose powder ad libitum. Consumption of this MCT powdered milk produced about eight to twenty fold increases in the serum 3-hydroxybutyrate concentrations.No significant anticonvulsant effects of ketogenic diet (MCT powdered milk) were observed against maximal electroshock seizure (20 mA×0.2 sec), pentetrazol (PTZ)-induced seizure (75 mg/kg), semicarbazide- induced seizure (100 mg/kg) or hydration electroshock seizure threshold (10 mA×0.2 sec) tests. However, a significant resistance was seen against the convulsion induced by the daily administration of subconvulsive dose of PTZ (45 mg/kg), which was a new experimental seizure model.Following daily administration of subconvulsive dose of PTZ i. p., mice with no seizure behavior developed minimal full seizures or clonic convulsions on the 4th day and finally some of them developed generalized tonic convulsions or death. Seven-week-old adult mice exhibited less protection against this seizure model than 4-week-old mice on ketogenic diet. Clonic convulsion induced by this new seizure model, the daily administration of subconvulsive dose of PTZ (45 mg/kg), was more intensive than that induced by a single injection of PTZ (75 mg/kg), when assessed by phenobarbital, phenytoin and sodium valproate. These results suggest that this new experimental seizure model is useful to assess the anticonvulsant properties of ketogenic diet.The contents of taurine, aspartic acid, valine, cystathionine, tyrosine, lysine and arginine were significantly low, and glutamine, alanine and cystine were significantly high in the brains of animals fed on ketogenic diet. No influence on the contents of GABA, glycine or glutamic acid by ketogenic diet was seen. A significant increase in brain noradrenaline content was observed with no increase in dopamine, serotonin or histamine in animals under ketogenic diet.