학술논문
THE EVALUATION OF AN ARTIFICIAL LIVER SYSTEM USING MONOLAYER CULTURE PIG HEPATOCYTES / 培養ブタ肝細胞を用いたハイブリッド型人工肝臓の機能評価
Document Type
Journal Article
Author
E HASHIMURA; F KUMAGAI; H MATSUE; J UCHINO; M TAKAHASHI; T HAMADA; T HASE; T KOMAI; T TSUBURAYA; Y NAKAJIMA; 中島 保明; 内野 純一; 圓谷 敏彦; 松江 弘一; 橋村 悦朗; 濱田 朋倫; 熊谷 文昭; 長谷 泰司; 駒井 喬; 高橋 学
Source
人工臓器 / Jinko Zoki. 1991, 20(1):156
Subject
Language
Japanese
ISSN
0300-0818
1883-6097
1883-6097
Abstract
A hybrid artificial liver(AFL) using monolayer cultured pig hepatocytes was evaluated in this paper. The module was constructed with 40 glass plates hepatocytes which were almost equivalent to 3.5-4.0g liver tissue in total. The glucose, urea and ammonia levels were 4.5 ug/hr/ Module, 22.0+4.2 ug/hr/Module, and constant for following 5 days. Hepatocytes in perfusion culture were almost maintained morphologically, for 5 days as well as in monolayer culture. These results indicate that our new AFL device using perfusion culture could support patients in hepatic failure.