학술논문

Data for method development of water droplet-in-oil digestion approach
Document Type
Journal Article
Source
Journal of Proteome Data and Methods. 2023, 5:1
Subject
Protein adsorption
Sample preparation
Single-cell proteomics
Water droplet-in-oil
Language
English
ISSN
2434-6454
Abstract
A water droplet-in-oil digestion (WinO) method was compared to an in-solution digestion (ISD) method to evaluate the improvement in the number and quantification of proteins in a single cell or trace samples. By using the WinO method, 400.3 ± 32.5 proteins were quantified from a single cell, whereas 140.8 ± 51.8 proteins were quantified by the ISD method. The recovery of proteins on the WinO method was 10.21-fold greater than the ISD method. The data described in this paper have been deposited to jPOST with the identifier JPST001390.