학술논문
Kit-positive interstitial cells pace-make in response to serotonin
Document Type
Journal Article
Source
Proceedings of Annual Meeting of the Physiological Society of Japan. 2007, :098
Subject
Language
English
Abstract
Serotonin (5-HT) is a key signaling molecule in both central and enteric nervous systems. We assessed The involvement of endogenous 5-HT receptors on pacemaker Ca2+ activity in gastrointestinal (GI). Cell cluster were prepared from the stomach and small intestine of WT mice (BALB/c), (including the enteric neurons) by enzymatic and mechanical treatments. After 2 days of culture, the fluo-3 AM (acetoxymethly ester of Fluo-3) was loaded to measure the intracellular Ca2+concentration [Ca2+]i. In the presence of nifedipine a dihydropyridine Ca2+ channel antagonist, spontaneous [Ca2+]i oscillations were observed within limited regions showing c-Kit-immunoreactivity, a marker of interstitial cells of Cajal (ICC). Application of 5-HT enlarged the region of pacemaker [Ca2+]i activity via type 3 5-HT receptors. RT-PCR and immunohistochemical examinations revealed expression of serotonin receptors in ICC. On the other hand, in cell cluster preparations from the stomach and small in intestine of W/Wv mice failed to respond to serotonin. The results indicated that Kit-positive interstitial cells respond to serotonin. [J Physiol Sci. 2007;57 Suppl:S98]