부산대학교 도서관

In this study, we crossed 31 basidiospore isolates from the fruit bodies of dikaryotic strain Grifola frondosa IM-BM21 to analyze mating compatibility groupings. Two incompatibility groups ( A1 and A2 ) were identified, identifying G. frondosa as a bipolar mushroom. Based on nucleotide sequences of whole genomic DNA of G. frondosa WM1-25 from next-generation sequencing, we designed oligonucleotide primers to amplify the homeodomain protein gene located on the A locus ( A1hox2 ) and pheromone genes ( A1phb1 ) located on the B locus in the tetrapolar mushroom. The DNA fragment of the A1hox2 gene was amplified only from A1 monokaryotic strains and the A1phb1 gene was amplified in all A1 and A2 monokaryotic strains. Therefore, the linkage groups of the analyzed genes were defined as A1hox2 in linkage group I, which is closely linked to A mating-type locus, and A1phb1 , which has no relationship with mating type.