부산대학교 도서관

Glycopeptides from the pronase digest of a 7 S protein have been separated into five fractions (peaks IIIa, IIIb, IIIc, IIId and IIIe) on Dowex 50-X 2 with 13.7mM pyridineacetic acid buffer (pH 3. 10). With the exception of the last peak (IIIe), the peaks were tripeptide-carbohydrates. All the fractions had asparagine as the N-terminal amino acid residue to which the carbohydrate moiety was attached. By amino acid sequence analysis, two tripeptides were found; Asp-Gly-Thr in peaks IIIa, IIIb and IIIc and Asp-Ala-Thr in peaks IIIb, IIIc and IIId. Peak IIIe was a mixture of three peptides. The tripeptides had two glucosamine and seven to nine mannose residues per aspartic acid, showing the same molar ratio as the L-β-aspartamido-carbohydrates. The same three types of carbohydrate groups probably resulted from paucidispersity.