학술논문
Rapid Multiplex RT-PCR for Influenza A and B by Genesoc®, a Microfluidic PCR System
Document Type
Journal Article
Author
Source
Yonago Acta Medica. 2023, 66(2):223
Subject
Language
English
ISSN
0513-5710
1346-8049
1346-8049
Abstract
Results The GeneSoC® assay detected the target sequences of influenza A and B at minimum concentrations of 38 and 65 copies/µL in reaction, respectively. For the analysis of clinical specimens, the positive, negative, and overall agreement between GeneSoC® RT-PCR and a conventional real-time RT-PCR was in all cases 100%, whereas for the comparison between GeneSoC® RT-PCR and the rapid antigen test, the agreements for positive, negative, and overall findings were 100%, 90.9%, and 95.7%, respectively. The mean time for completing GeneSoC® RT-PCR was 16 min 29 s (95% confidence interval, 16 min 18 s to 16 min 39 s).