학술논문
Characterization of the 16S-23S rRNA Intergenic Spacer Regions among Strains of the Fusobacterium necrophorum Cluster
Document Type
Journal Article
Author
Source
Journal of Veterinary Medical Science. 2002, 64(3):273
Subject
Language
English
ISSN
0916-7250
1347-7439
1347-7439
Abstract
The 16S-23S rRNA intergenic spacer regions (ISRs) of Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme were characterized. Products of two sizes, about 360 bp (small) and 530 bp (large), were generated by PCR amplification from the 16S-23S rRNA ISR of all the strains tested. The large and small 16S-23S rRNA ISRs of F. necrophorum exhibited a level of sequence similarity of 93.9% to 99.7% and 94.2% to 98.6% homologies within the species, respectively. Only the large spacer regions in these bacteria contained one or two tRNA genes. F. necrophorum subsp. necrophorum contains the isoleucine and alanine tRNA gene, whereas F. necrophorum subsp. funduliforme contains the isoleucine tRNA gene.