부산대학교 도서관

For the detection of mutations occurring in somatic or germ cells in vivo, specific locus tests, or transgenic mouse systems carrying exogenous marker gene have been adopted. However, they have limitations in the tissues to be examined or in detectable size of deletion mutations. To improve the limitations, we plan to develop new assay systems that use GFP for mutant detection. The principle of the approach is to make the cells fluorescent when mutation occurred at specified gene loci in the genome. For example, co-expression of two vectors, one produces GFP constitutively and the other produces repressor protein to silence the expression of GFP gene. In this system, any kinds of forward mutation in the repressor gene allow GFP gene transcription from null state, which should make the mutant cells easy to be recognized by green fluorescence. Toward the generation of mouse model systems, we have been evaluating the systems in cultured cells.