학술논문
Arabidopsis Encyclopedia using Full-length cDNAs and its Application for Functional Genomics / シロイヌナズナ完全長cDNAエンサイクロペディアの作製と遺伝子の機能・発現解析への利用
Document Type
Journal Article
Author
Akiko Enju; Asako Kamiya; Athanasios Theologis; Ayako Kamei; Jong-Myong Kim; Joseph R. Ecker; Jun Kawai; Junko Ishida; Kazuko Yamaguchi-Shinozaki; Kazuo Shinozaki; Kei Iida; Kenji Akiyama; Maiko Nakajima; Mari Narusaka; Masakazu Satou; Miki Fujita; Motoaki Seki; Piero Carninci; Ronald W. Davis; Saho Mizukado; Shigeyuki Yokoyama; Taiko To; Taishi Umezawa; Tatsuya Sawasaki; Tetsuya Sakurai; Tokihiko Nanjo; Yaeta Endo; Yoshihide Hayashizaki; Yoshinori Hasegawa; Youko Oono; 中嶋 舞子; 亀井 綾子; 佐藤 将一; 大野 陽子; 林崎 良英; 梅澤 泰史; 楠城 時彦; 槐 亜希子; 横山 茂之; 櫻井 哲也; 水門 佐保; 河合 純; 澤崎 達也; 石田 順子; 神谷 麻子; 秋山 顕治; 篠崎 一雄; 篠崎 和子; 藤 泰子; 藤田 美紀; 遠藤 弥重太; 金 鍾明; 長谷川 嘉則; 関 原明; 飯田 慶; 鳴坂 真理
Source
Plant and Cell Physiology Supplement. 2003, :86
Subject
Language
Japanese
Abstract
Full-length cDNAs are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. Using the biotinylated CAP trapper method, we constructed full-length cDNA libraries from Arabidopsis plants and isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. They were clustered into 14,668 nonredund ant cDNA groups, about 60% of predicted genes1). We have determined full-length cDNA sequences of 12,189 RAFL cDNA clones as of Nov. 29, 2002. We have also used the RAFL cDNAs for the microarray analysis of expression profiles of Arabidopsis genes under drought, cold and high-salinity-stresses2),3),4). In this meeting, overview of our RAFL cDNA project will be presented.1) Seki et al. (2002) Science 296:141-145. 2) Seki et al. (2001) Plant Cell 13:61-72. 3) Seki et al. (2002) Plant J. 31:279-292. 4) Seki et al. (2002) Funct. Integ. Genomics 2:282-291.