부산대학교 도서관

Objective : We studied the origin of orangeophilic cells (OPCs) because such cells reported to be present in body-cavity fluids in malignant mesothelioma.Study Design : The subject was one case of epoxy-embedded samples of malignant mesothelioma diagnosed at the Yamaguchi Grand Medical Center containing OPCs in smear samples. We consecutively prepared semi-thin sections (4.0μm) and ultra-thin sections (80 nm) from epoxy-embedded blocks. We removed epoxy resin from semi-thin sections and conducted Papanicolaou staining, microscopically observing OPCs. We also applied conventional electron staining to ultra-thin sections next to semi-thin sections, ultrastructurally observing OPCs in contrast to micrographs.Results : Ultrastructurally, OPCs had microvilli, and intermediate filaments indicated that the origin was malignant mesothelioma. Malignant mesothelioma cells and OPCs had lipid droplets in cytoplasm.Conclusions : Ultrastructural examination indicated that OPCs were derived from malignant mesothelioma or mesothelial cells. These results suggest that OPCs may be an indicator of malignant mesothelioma.