부산대학교 도서관

Since cellular adhesion and spreading is regulated by protein adsorption, patterning of proteins responsible for cellular adhesion leads to spatially directed cellular adhesion. In this study, micropatterned surfaces were prepared by coating of mercapto-functionalized poly(ethylene glycol) (PEG) on Au surface which wroks for repelling proteins, followed by plasma-etching through a metal mask pattern with circular holes. Then the micro-patterning of bovine articular chondrocytes hetero-spheroids underlaid with human umbilical endothelial cells (HUVEC) was achieved. SPR study showed that PEG chain density play a critical role to inhibit protein adsorptions, and consequent formation of spheroids showed good accordance to this SPR data. These arrayed spheroids are promising materials for tissue and cell based biosensors as well as tissue engineering.