부산대학교 도서관

When the mushroom tyrosinase was incubated with p-octylphenol (p-OP), p-nonylphenol (p-NP), and bisphenol A (BPA) at 37°C, it oxidized p-OP but not p-NP and BPA. The optimal pH and temperature for the enzymatic oxidation of p-OP were 6.0 and 35-40°C, respectively. Michaelis constant of the mushroom tyrosinase for p-OP was 0.17mM (Oxygen uptake method) and 0.63mM (Absorbance method), and it was similar to that for p-cresol. More than 95% of p-OP was oxidized for 10min by mushroom tyrosinase, slthough octyldiphenol was formed as an intermediate during the enzymatic oxidation of p-OP.