학술논문
In vitro model for axonal injury and functional recovery in microengineered human iPSC-derived neuronal networks / 微細加工技術を用いたヒトiPS細胞由来神経回路モデルの構築、および軸索損傷後の接続性回復評価への応用
Document Type
Journal Article
Author
Source
Annual Meeting of the Japanese Society of Toxicology. 2020, :P-135
Subject
Language
Japanese
Abstract
Several types of neuronal populations were generated by transcription factor-induced differentiation of iPSCs and were shown to exhibit specific and reproducible spontaneous burst profiles when cultured individually. Then, using micropatterning processes, distinct neuronal populations were arranged in separate areas of a MEA. Co-cultured neuronal clusters synchronized their firing activity over time, indicating that synaptic connections occurred both within and among the neuronal clusters. These micropatterned structures were further evaluated for their response to neurotransmitter receptor blockers and for the functional recovery of connectivity following axonal injury.