학술논문
Quantitative Analysis for Water-soluble Porphyrin Derivatives Adsorbed in Saccharomyces cerevisiae by Absorption Spectrophotometry Using Confocal Laser Scanning Microscope / 共焦点レーザー走査型顕微鏡を用いる酵母菌体へ吸着した水溶性ポルフィリン誘導体の吸光光度定量
Document Type
Journal Article
Author
Source
分析化学 / BUNSEKI KAGAKU. 2009, 58(5):357
Subject
Language
Japanese
ISSN
0525-1931
Abstract
An evaluation of the bio-affinity of methoxo(alkyloxo)- tetraphenylporphyrinatoantimony bromide (1) was examined by an absorption experiment of 1 toward Saccharomyces cerevisiae. An aqueous solution of 1 was added to an aqueous solution of S. cerevisiae. Then an aqueous solution of agar was added into the solution in order to stop the Brownian motion of bacteria during analysis. A prepared aqueous solution containing 1(10 μmol dm−3), S. cerevisiae (1.0×104 cells cm−3) and agar (0.4 wt%) was taken inside a space surrounded by a silicone spacer (1 cm×1 cm, thickness 50 μm) put on a slide glass, which was set on a confocal laser scanning microscope (CLSM) stage. By measuring the diameter of the cell and the absorbance on CLSM, the concentrations of 1 adsorbed inside S. cerevisia were determined to be 54.2±7.3 for 1a, 49.8±6.2 for 1b, and 22.5±15.5 mmol dm−3 for 1c according to Lambert-Beer`s law.