학술논문

Comprehensive Phenotypic Analyses of Transgenic Rice that Express the Chimeric Repressor / イネ転写因子キメラリプレッサー過剰発現イネ系統の網羅的表現型解析
Document Type
Journal Article
Source
Plant and Cell Physiology Supplement. 2011, :0002
Subject
Language
Japanese
Abstract
Transcription factors (TFs), which regulate the first step of the gene expression, affect various biofunction. Therefore, elucidation of the function of each TF will lead to identify the relationship between genes and the biofunction that is regulated by a gene. However, gene redundancy of TFs often interferes such efforts. We have developed a novel gene silencing technology (CRES-T), which can overcome the gene redundancy and induces loss-of-function phenotype of TF by expressing chimeric repressor of the TF. Using CRES-T, we have clarified function of various TFs in Arabidopsis thaliana, a model plant in dicot. In this study, we are applying the CRES-T to rice, a model plant in monocot, using rice full-length TF cDNAs. We have created 314 kinds of transgenic rice that express chimeric repressor of nine TF families including bZIP, Myb, NAC and others. We here report intriguing phenotypes and comparative analysis of TFs between rice and Arabidopsis.

Online Access