부산대학교 도서관

Peripheral blood stem cells are important for stem cell transplantation. Purification of tissue stem cells from bone marrow is also useful for therapy in tissue engineering. Here we report blood cell separation through surface-modified polyurethane forming membranes. Several functional groups (i.e., -SO3H, -OH, -COOH, -NH2, N(C2H5)2, -NHC2H4OH) were introduced on epoxied polyurethane forming membranes. The permeation of peripheral blood through the surface-modified membranes was investigated. T and B cells were almost perfectly removed from the blood through the surface-modified membranes. CD34+ cells (Peripheral blood stem cells) were also removed from the blood through the surface-modified membranes. When elusion solution of 0.5 wt% albumin was permeated through the membranes after the permeation of blood, CD34+ cells could be recovered through the membranes containing -COOH group. It is concluded that specific blood cells can be separated and recovered from the permeation of peripheral blood through the surface-modified polyurethane forming membranes.