학술논문
THE COMBINATION EFFECTS OF ANTIBACTERIAL AGENTS AGAINST CLINICAL ISOLATED MULTIPLE-DRUG RESISTANT Pseudomonas aeruginosa / 臨床分離薬剤耐性緑膿菌における各種抗菌薬の併用効果の検討
Document Type
Journal Article
Author
GIICHI HASHIKITA; KAZUMASA SASAKI; MASAHARU WATANABE; MITSUAKI NAGASAWA; NORIYASU SUZUKI; SADAO TAKAYAMA; SAYOKO KAWAKAMI; SHIGEFUMI MAESAKI; SHUNSUKE SHIBUYA; SUGAKO KOBAYASHI; TADASHI OKABE; TAKASHI UCHIDA; TOSHIYUKI YAMAGUCHI; 佐々木 一雅; 内田 幹; 前暗 繁文; 小林 清子; 山口 敏行; 岡部 忠志; 川上 小夜子; 橋北 義一; 渋谷 俊介; 渡邊 正治; 鈴木 憲康; 長沢 光章; 高山 貞男
Source
The Japanese Journal of Antibiotics. 2006, 59(1):11
Subject
Language
Japanese
ISSN
0368-2781
2186-5477
2186-5477
Abstract
The effectiveness of antibacterial agents against 70 strains of clinically isolated multiple-drug resistant Pseudomonas aeruginosa (MDRP) was measured by the micro dilution method. Fifty of all strains (71%) produced metallo-β-lactamase and the IMP-1 gene was detected by polymerase chain reaction (PCR). The MIC90 (the minimum inhibitory concentration of an antibiotic necessary to inhibit the growth of 90% of bacterial strains) values of biapenem (BIPM), meropenem (MEPM), tazobactam/piperacillin (TAZ/PIPC), sulbactam/cefoperazone (SBT/CPZ), cefepime (CFPM), ciprofloxacin (CPFX), pazufloxacin (PZFX), amikacin (AMK) and aztreonam (AZT) were found to be 265, 512, 256, 512, 512, 64, 128, 128 and 128 μg/mL, respectively. The in vitro combination effects of antibacterial agents were examined against 62 strains of MDRP and the synergy or additive effects were evaluated by fractional inhibitory concentration (FIC) index calculated by the checkerboard method. The combination of AMK and AZT showed synergy effects on 15/59 (25.4%) strains of MDRP. The synergy and additive effects on the MDRP strains were also found by the other antibacterial agents combination such as TAZ/PIPC and AMK, CFPM and AMK, and SBT/CPZ and AZT. These results suggested the necessity of further investigation of clinical usefulness.